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. Author manuscript; available in PMC: 2010 May 15.
Published in final edited form as: Dev Biol. 2009 Feb 27;329(2):191–200. doi: 10.1016/j.ydbio.2009.02.020

Fig. 1.

Fig. 1

Knockdown of Hand2 by siRNA. (A) Western blot analysis of Hand2 expression in a chick DF-1 cell line with permanent expression of Hand2-myc. DF-1 cells were transfected with control siRNA directed against GFP and NP25 and with two different siRNAs directed against ggHand2. Protein extracts were subjected to PAGE electrophoresis and analyzed by western blotting for Hand2 and β-actin. (B) The intensity of Hand2 protein bands were quantified and normalized to β-actin. Hand2 expression levels were compared to siGFP control transfections. Hand2 siRNA1 and Hand2 siRNA2 reduced Hand2 protein levels to about 40% and 50% of control, respectively, whereas NP25 siRNA had no effect. **P<0,05.