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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Nat Immunol. 2009 Aug 16;10(9):958–964. doi: 10.1038/ni.1775

Figure 5.

Figure 5

Signaling events mediating the activation of PARP-1 by 15-HC. (a) Time course of microglial PARP-1 activation by 15-oxysterols or 7-KC. P value, two-way ANOVA. (b) Signaling pathways activated in microglial cells stimulated with 15-HC and lysed at 1, 5 or 15 min; signal-transduction arrays prepared with the lysates were probed with phosphorylation-specific antibodies. Phosphorylated (p-) residues are in parentheses on vertical axes. P values, one-way ANOVA. (c) Activation of microglial PARP-1 by 15-HC in the presence of kinase inhibitors. P values, one-way ANOVA. (d) Coimmunoprecipitation of phosphorylated Erk1/2 and PARP-1 in lysates of microglial cells treated with PBS (control) or 15-HC, immunoprecipitated (IP) with antibodies to phosphorylated Erk1/2 and then analyzed by immunoblot (IB) with anti-PARP-1. Below, immunoblot analysis of whole-cell lysates with anti-PARP-1 (loading control). Right, ratio of PARP-1 immunoprecipitated with antibody to phosphorylated Erk1 (p-Erk–PARP-1) to total PARP-1 (t-PARP-1) in lysates. Data are representative of two (b) or three (a,c,d) independent experiments (mean and s.e.m., a–c).