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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Nat Immunol. 2009 Aug 16;10(9):958–964. doi: 10.1038/ni.1775

Figure 6.

Figure 6

Activation of PARP-1 by 15-HC is mediated by TLR2. (a) PARP-1 activity in microglia, macrophages and astrocytes activated with 15-HC in the presence of blocking anti-TLR2 or anti-TLR4 or isotype-matched control antibody (IC). (b) PARP-1 activity in HEK293 cells left untransfected (293-null) or transfected with plasmid encoding TLR2 (293-TLR2), then activated with 15-HC or with LPS from Porphyromonas gingivalis (pgLPS) or Escherichia coli (ecLPS). (c) Disease scores of TLR2-deficient (TLR2-KO) and wild-type mice treated with PBS or 15-HC during the induction and course of EAE. (d) PARP-1 activity in CD11b+ CNS cells isolated from TLR2-deficient and wild-type mice treated with PBS or 15-HC during the course of EAE. P values, test. (e) Expression of TNF, iNOS and CCL2 mRNA by CD11b+ CNS cells isolated from TLR2-deficient and wild-type mice treated with PBS or 15-HC during the course of EAE. P values, one-way ANOVA (a,b,d,e) or two-way ANOVA (c). Data are representative of three independent experiments (mean and s.e.m.).