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. 2008 May 9;2:81–112.

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© 2009 The authors.

This article is published under the Creative Commons Attribution By licence. For further information go to: http://creativecommons.org/licenses/by/3.0.

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Dose dependence of the haemolytic activity of tolaasin I (circles) and WLIP (squares) after 4 hours at room temperature. Dotted lines are best fit experimental data points with Hill equation. Hill coefficients are 6.3 and 8.0 for tolaasin I and WLIP, respectively. Inset: Set of traces recorded on the microplate reader of kinetics of haemolysis of RBC exposed to 2 step sequential dilutions of tolaasin I (left) and WLIP (right), from top to bottom. Starting concentration (top panels) were 100 μg/ml for tolaasin I and 6.25 μg/ml for WLIP. The decrease of turbidity over time indicated the disappearance of intact RBC. All panels have the same linear axis, ranging from 0 to 4 hours and from 0 to 110 mOD for x- and y-axes, respectively.