Figure 3.

Heat shock suppresses gene expression of VEGF variants in HT-1080 cells. Confluent HT-1080 cells were treated with or without heat shock at 42°C for 4 h and then incubated for another 24 h. Isolated RNA was subjected to RT–PCR analysis with 27 cycles for VEGF₁₂₁ and VEGF₁₆₅ and with 29 cycles for VEGF₁₈₉ as described in Figure 2. The relative amounts of VEGF mRNA were quantified by densitometric scanning followed by normalising against that of GAPDH mRNA and expressed taking the untreated HT-1080 cells as 100. Three independent experiments were reproducible and typical data were shown.