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. 2002 May 3;86(10):1592–1596. doi: 10.1038/sj.bjc.6600269

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Copyright © 2002 Cancer Research UK

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Western blot. The 7 base pair mutation was cloned into the mammalian expression plasmid pC53-SN3, which was transfected into Saos-2 cells. A Western blot was carried out using lysates from these cells, cells transfected with WT p53 and PHA stimulated lymphocytes from the patient. The blot showed WT p53 with the expected size of 53 kDa (lane 1) and the p53 from the patient's lymphocytes also at size 53 kDa (lane 2). The cells transfected with the manufactured mutation showed a protein on the blot at about size 27 kDa (lane 3).