Table 1.

TEMPORAL EXPRESSION OF WNTs IN MURINE EMBRYONIC SECONDARY PALATAL TISSUE

Gene	E13.5	E14.5
Wnt-2	2.0 ± 0.3	3.0 ± 0.5
Wnt-4	1.1 ± 0.1	2.2 ± 0.3
Wnt-10a	4.0 ± 0.9	5.6 ± 0.4
Wnt-10b	1.5 ± 0.4	3.1 ± 0.6
Wnt-16	3.8 ± 0.5	3.2 ± 0.5

Real-time PCR was performed on cDNAs generated from RNA purified from microdissected murine embryonic palatal tissue on E12.5, E13.5, and E14.5 using primers for the indicated gene. Signals were normalized to that from GAPDH. Fold-change was calculated using the relationship, fold change = 2^−ΔΔCt. Data presented represent fold-increases in expression (relative to E12.5) ± standard deviation (n=3–4).