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. Author manuscript; available in PMC: 2010 Feb 20.
Published in final edited form as: Nature. 2009 Aug 20;460(7258):1016–1020. doi: 10.1038/nature08236

Figure 4. Transmitter release regulates synapse formation but not elimination, causing a gradual divergence of synaptic development between wildtype and mGluR6-YFP/TeNT mice.

Figure 4

a, b, Developmental increase in density (a) and number (b) of PSD95-CFP puncta on ON RGCs (5 -16 cells per time point and genotype). c, First image of a time-lapse series of a P9 RGC labeled with td-Tomato (blue) and glutamatergic postsynaptic densities labeled with PSD95-CFP (red). d, Time series of the region indicated by a yellow box in c in which a PSD95 cluster forms (arrow). YFP expression in BCs shown in green. PSD95-CFP channel isolated in the lower panels. e, Higher magnification of a single plane of the region indicated by the white box in d showing that the new punctum formed at an apposition of BC axon and RGC dendrite. f - h, analogous to c - e for loss of a PSD95 cluster. i, j, Summary data (mean ± s.e.m) for the rate of PSD95-FP cluster formation (i) and elimination (j). * indicates p < 0.05.