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. Author manuscript; available in PMC: 2010 Jul 16.
Published in final edited form as: Neuron. 2009 Jul 16;63(1):48–62. doi: 10.1016/j.neuron.2009.06.006

Figure 2. Loss of Fgf10 delays radial glia differentiation.

Figure 2

(A–F’) Immunofluorescence staining of wt (wt) (A–F) and Fgf10 −/− (A’–F’) brains at ages indicated with antibodies against radial glia markers, BLBP (A–E’) and GLAST (F, F’). At E11.5 in wt, stronger staining for BLBP and GLAST was detected in medial and ventrolateral cortex (arrowheads in A, F) and moderate staining elsewhere in cortex (arrow). Same staining pattern is evident at E12.5, but overall staining levels are higher (B). By E13.5, staining becomes relatively high throughout cortex, and the radial glia fibers emanating from the pallial-subpallial boundary are particularly intensely labeled (arrow in C). Compared to wt, in the Fgf10 −/− cortex, BLBP staining (A’, B’) and GLAST staining (F’) is substantially diminished. By E13.5, staining is comparable between wt (C) and Fgf10 −/− cortex (C’). Arrowheads and arrows mark same relative positions in Fgf10 −/− cortex (A’–C’) as in wt cortex (A–C). Moderate BLBP staining in mid cortex of wt at E11.5 and E12.5 (arrow in A, B, respectively) is shown at higher magnification in D and E. Diminished BLBP staining in mid cortex of Fgf10 −/− cortex at E11.5 and E12.5 (arrow in A’, B’, respectively) is shown at higher magnification in D’ and E’. Scale bars: 0.5 mm (A, A’), 0.5 mm (B, B’), 0.5 mm(C, C’), 50 µm (D–F’), 0.5 mm (F, F’). Ctx: cortex, GE, ganglionic eminence, VZ ventricular zone, PP: preplate.