Table 3.
Peripheral tissues and cell types other than endothelium exhibiting positive labeling for PDE2A
| Tissuea | Component | Rat | Mouse | Dog | Human | Cynomolgus monkey | Staining pattern |
|---|---|---|---|---|---|---|---|
| Kidney | Tubular epithelium | 1+ | (−) | 1+ | 1+ | (−) | Rat, dog, human; cytoplasm of tubular epithelia cells |
| Lung | Individual cells | 3+ | 1+ | (−) | (−) | 1+ | Rat; round cells on surface of alveoli; cynomolgus monkey and mouse; bronchial epithelium |
| Ovary | Epithelium | 1+ to 2+ | 2+ | 1+ | (−) | 1+ to 3+ | Rat, mouse, dog, cynomolgus monkey; corpora lutea and follicular epithelium |
| Pituitary | 2+ | 2+ | 2+ | NE | (−) | Rat, mouse, dog; predominantly neuropil staining of pars nervosa with rare cells in pars distalis | |
| Adrenal | 3+ | 3+ | 3+ | 2+ to 3+ | 3+ | Strong cytoplasmic staining in all species | |
| Epididymis | Smooth muscle; interstitium | 3+ | 1+ | (−) | NE | (−) | Rat; interstitial macrophages; mouse; light staining of smooth muscle cells |
| Lymph node | Lymphocytes/macrophages | 2+ | 2+ to 3+ | 1+ | (−) | 1+ to 3+ | Rat, mouse, dog; lymphocyte subsets; cynomolgus monkey; macrophages |
| Dorsal root ganglion | 1+ to 3+ | 1+ to 2+ | 1+ to 2+ | NE | 1+ | Cytoplasmic and nuclear staining of subsets of neurons | |
| Urinary bladder | Urothelium | 1+ | 1+ to 2+ | (−) | (−) | NE | Rat, mouse; cytoplasm of epithelial cells |
| Smooth muscle | 1+ | 1+ to 2+ | (−) | (−) | NE | Rat and mouse; smooth muscle of bladder wall | |
| Tongue | Epithelium | 1+ | (−) | (−) | NE | (−) | Rat; stratum spinosum |
| Uterus | Epithelium | (−) | (−) | (−) | 1+ to 2+ | (−) | Human; cytoplasmic staining of endometrial epithelium |
| Testis | Seminiferous tubules | 1+ | 1+ | (−) | 2+ | 1+ to 2+ | Rat, mouse, human, and cynomolgus monkey; subsets of spermatogenic and Sertoli cells |
| Interstitium | (−) | (−) | (−) | 1+ to 2+ | 1+ | Human, cynomolgus monkey; occasional cytoplasmic staining of interstitial cells | |
| Prostate | Epithelium | 1+ | (−) | (−) | 1+ to 2+ | 1+ | Rat, human; prostatic epithelium; cynomolgus monkey; ganglionic neurons, not epithelium. |
| Stomach | Epithelium | 1+ to 3+ | 1+ to 2+ | 1+ | 2+ to 3+ | 2+ | Cytoplasmic staining of epthelial cells in the gastric pits of all species |
| Ganglia | 2+ to 3+ | 2+ | (−) | NE | 2+ | Rat, mouse, cynomolgus monkey; neurons of myenteric plexi (ganglia) | |
| Small intestine | Epithelium | 1+ to 2+ | 1+ | 1+ to 2+ | 1+ to 2+ | (−) | Cytoplasmic staining of mucosal epithelium in all species except cynomolgus monkey |
| Ganglia | 2+ to 3+ | 2+ to 3+ | 1+ | NE | 1+ to 2+ | Rat, mouse, dog, cynomolgus monkey; neurons of myenteric plexi (ganglia) | |
| Cecum | 2+ to 3+ | 1+ to 2+ | 1+ | NE | 1+ to 2+ | Rat, dog, and cynomolgus monkey; neurons of myenteric plexi (ganglia); mouse; basal epithelium | |
| Colon | Epithelium | 3+ | 1+ to 2+ | 1+ to 2+ | 1+ | (−) | Cytoplasmic staining of mucosal epithelium in all species except cynomolgus monkey |
| Ganglia | 2+ to 3+ | 2+ to 3+ | 1+ | 1+ to 2+ | 1+ to 2+ | All species; neurons of myenteric plexi (ganglia) | |
| Pancreas | Islets | 1+ | (−) | 1+ | 2+ to 3+ | (−) | Rat, dog, human; cytoplasmic staining of islet cells |
| Ganglia | 1+ | 1+ to 2+ | 1+ to 2+ | NE | NE | Rat, mouse, dog; neuronal cytoplasm and nuclei | |
| Salivary gland | Ganglia | (−) | (−) | (−) | NE | 2+ | Cynomolgus monkey; cytoplasm of neurons |
| Trachea | Epithelium | 1+ to 3+ | (−) | 2+ | NE | 2+ | Rat, dog, and cynomolgus monkey; epithelium |
| Spleen | Lymphocytes | 1+ to 2+ | 1+ to 2+ | 1+ | (−) | (−) | Rat, mouse, and dog; lymphocytes of red and white pulp |
| Thymus | Individual cells | (−) | 2+ | (−) | (−) | (−) | Mouse; rare round to polygonal cells in cortex and medulla |
| Thyroid | Epithelium | (−) | (−) | (−) | 1+ to 2+ | (−) | Human; cytoplasmic staining of follicular epithelium |
| Mammary gland | Epithelium | (−) | (−) | (−) | 2+ | (−) | Human; cytoplasmic staining of ductal epithelium |
| Eye | Ciliary epithelium | 1+ | 2+ to 3+ | 1+ | NE | (−) | Rat, mouse, dog; cytoplasm of epithelial cells and some smooth muscle cells |
| Retina | 1+ to 2+ | 2+ to 3+ | 1+ | 1+ to 3+ | Rat, mouse; cytoplasmic staining of rare cells in the inner nuclear cell layer; cynomolgus monkey and dog; nuclei of ganglion cell layer | ||
| Skin | Epithelium | (−) | (−) | 1+ | 2+ | 3+ | Dog, cynomolgus monkey; apocrine gland epithelium; human; basal layer of epidermis |
| GALT or BALT | 1+ | 1+ to 3+ | (−) | (−) | 1+ | Rat GALT; mouse GALT and BALT; cynomolgus monkey GALT |
a
There is PDE2A staining of endothelium in most tissues from all species, seen consistently in veins and capillaries and in subsets of arteries.
NE, tissue not evaluated; −, negative; 1+, mild staining of tissue components; 2+, moderate staining of tissue components; 3+, marked staining of tissue components; GALT, gut-associated lymphatic tissue; BALT, bronchial-associated lymphatic tissue.