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. Author manuscript; available in PMC: 2009 Sep 18.

Published in final edited form as: Circulation. 2009 Apr 20;119(17):2357–2366. doi: 10.1161/CIRCULATIONAHA.108.814145

miRNA expression in the I/R hearts. (A-C) Occlusion of the left anterior descending coronary artery (LAD) for 30 min, followed by 24-h reperfusion (I/R), induced cardiac injury (infarction and apoptosis) in mice. (A) Infarct zone was observed in I/R hearts (white-gray zone, indicated by arrows), but not in sham samples. (B) The number of apoptotic nuclei and (C) the degree of DNA fragmentation were greatly increased in I/R hearts, compared with the shams (n=6, * P<0.01). (D) A partial heat-map of the upregulated and downregulated miRNAs (labeled by arrows). All of the microRNA array raw data is available in the Online supplemental data. (E) Dysregulated expression of miRNAs was confirmed by Taq^man RT-PCR (normalized to control snoRNA412). RT-PCR primer sets for these miRNAs and control snoRNA412 were purchased from Ambion, Inc. We did not validate ambi-miR-9651 due to no available primer sets for this miRNA. (n=6, * P<0.05).

miRNA expression in the I/R hearts. (A-C) Occlusion of the left anterior descending coronary artery (LAD) for 30 min, followed by 24-h reperfusion (I/R), induced cardiac injury (infarction and apoptosis) in mice. (A) Infarct zone was observed in I/R hearts (white-gray zone, indicated by arrows), but not in sham samples. (B) The number of apoptotic nuclei and (C) the degree of DNA fragmentation were greatly increased in I/R hearts, compared with the shams (n=6, * P<0.01). (D) A partial heat-map of the upregulated and downregulated miRNAs (labeled by arrows). All of the microRNA array raw data is available in the Online supplemental data. (E) Dysregulated expression of miRNAs was confirmed by Taq^man RT-PCR (normalized to control snoRNA412). RT-PCR primer sets for these miRNAs and control snoRNA412 were purchased from Ambion, Inc. We did not validate ambi-miR-9651 due to no available primer sets for this miRNA. (n=6, * P<0.05).