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. Author manuscript; available in PMC: 2009 Sep 18.
Published in final edited form as: Circulation. 2009 Apr 20;119(17):2357–2366. doi: 10.1161/CIRCULATIONAHA.108.814145

Figure 3.

Figure 3

Overexpression of miR-320 increased I/R-induced cardiac injury in vivo. (A) Diagram of miR-320 TG construct. Transgenic mice were generated using a mouse primary miR-320 DNA under the control of the α-myosin heavy chain promoter (α-MHCp). (B) Northern blots showed that mature miR-320 was overexpressed in the transgenic hearts. Loading control was performed with an RNA probe against the small housekeeping RNA, U6 (106 nt). (C-D) miR-320 overexpression greatly increased myocardial infarct size after 30 min myocardial ischemia, via left anterior descending (LAD) coronary artery occlusion, followed by 24-h reperfusion, (E) while the region at risk was not significantly different among groups. (n=8, WT; n=6, miR-320 TG. *P<0.05 vs WT).