Figure 7.
In vivo effects of antagomir-320 administration on myocardial ischemia/reperfusion. (A) The levels of miR-320 expression were determined in murine hearts 3 days after administration of antagomir-320, antagomir-320 mutant and saline control by Taqman RT-PCR, as described in Figure 1 legend. (B) Western blot and densitometric analysis of Hsp20 expression in murine hearts 3 days after treated with these anatgomirs. Calsequestrin (CSQ) was used as a loading control. (C) Antagomir-320 treatment greatly reduced myocardial infarct size after 30 min myocardial ischemia followed by24-h reperfusion, while the region at risk was not significantly different among groups. (n=7, saline; n=5, mutant antagomir-320; n=7, antagomir-320. *P<0.05 vs saline control).