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. Author manuscript; available in PMC: 2010 Sep 1.

Published in final edited form as: J Mol Cell Cardiol. 2009 Jun 6;47(3):382–390. doi: 10.1016/j.yjmcc.2009.05.016

Cardiac-specific expression of Hsp20 inhibits NF-κB activation. (A) Representative autoradiographs of an EMSA for NF-κB, and (B) Image analysis of activity of NF-κB. Results are representative of 3 separate time course experiments. *P < 0.05 vs. NTG mice. C) Representative Western blot analysis of phosphorylated and total IκBα in NTG and Hsp20-TG mice with or without LPS treatment (25μg/g) for 30 min. α-Actin was used as a loading control. D) Image analysis of phosphorylated and total IκBα determined by densitometry. Fold increase was calculated vs. respective NTG value (vehicle) set to 1.0. *P < 0.001, # P < 0.05 vs. vehicle mice, n=4 per group.

Cardiac-specific expression of Hsp20 inhibits NF-κB activation. (A) Representative autoradiographs of an EMSA for NF-κB, and (B) Image analysis of activity of NF-κB. Results are representative of 3 separate time course experiments. *P < 0.05 vs. NTG mice. C) Representative Western blot analysis of phosphorylated and total IκBα in NTG and Hsp20-TG mice with or without LPS treatment (25μg/g) for 30 min. α-Actin was used as a loading control. D) Image analysis of phosphorylated and total IκBα determined by densitometry. Fold increase was calculated vs. respective NTG value (vehicle) set to 1.0. *P < 0.001, # P < 0.05 vs. vehicle mice, n=4 per group.