(A) Subcellular localization of EGFP-tagged ACL in LN229 cells imaged by deconvolution microscopy. EGFP-ACL, visible in both nucleus and cytoplasm, is contrasted to DsRed mitochondrial marker, which is excluded from the nucleus. DAPI staining of nuclear DNA is restricted to the nucleus. (B) Western blot analysis of cytoplasmic and nuclear protein extracts from HCT116 cells. Quantitation represents mean +/- SD from 4 [ACL, AceCS1] or 8 [PARP, tubulin] nuclear and cytoplasmic samples. ACL and AceCS1 are both significantly (***, p<0.0001) enriched in the nuclear fraction as compared to tubulin.