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. Author manuscript; available in PMC: 2010 Jan 16.
Published in final edited form as: Nature. 2009 Jun 24;460(7253):396–399. doi: 10.1038/nature08130

Figure 2. WWP-1 ubiquitin ligase activity is essential for DR induced longevity.

Figure 2

a, Mutation of the conserved catalytic cysteine of WWP-1 abolishes ubiquitin ligase activity. In vitro ubiquitination assay of recombinant WT WWP-1 or mutant WWP-1 (C762A) using C. elegans embryo extract. b, eat-2(ad116) mutant worms expressing a dominant negative wwp-1(C762A) have significantly shorter lifespans than control worms expressing GFP. Lifespan values are given in Supplementary Table 1.