Fig 4.

Protein domains and localization of Num1. A) Domain architecture of Num1. The amino-terminal coiled-coil (CC) was identified using the secondary structure prediction program, Coils. The tandem repeat region consists of twelve repeats of 64 amino acids [Verstrepen et al., 2005]. The function of this region is not known. The PH domain was confirmed by a genome-wide analysis of PH domains in budding yeast [Yu et al., 2004]. B) The PH domain is necessary and sufficient to target Num1 to the plasma membrane. Localization of full-length Num1, a truncation that lacks the PH domain (ΔPH), and the PH domain alone. Each allele is tagged with RFP and expressed from the endogenous chromosomal locus (Moore, unpublished).