Effect of ECS on NGF and TrkA immunoreactivity in the paraventicular (PV) and reuniens (Re) thalamic nuclei. Representative photographs illustrate NGF (A–D) and TrkA (E–H) immunoreactivity in sham treated control animals (A, C, E, and D), and at 7 hr after chronic ECS treatment (B, D, F, and H). Histograms show the mean values (for each groups of animals; n=6) of the number of NGF or TrkA immunopositive cells after acute (striped bars) or chronic ECS (solid bars), as compared to control (sham ECS) group (open bars). Asterisks indicate significant difference (*P<0.05 ; **P<0.01; ***P<0.001; ANOVA with Fisher’s test) from control group. The black boxes in the schematic brain section illustrate the areas from which the photographs and measurements were taken. Photographs were acquired at 10x (A – F) and 40x magnification (A’ – F’).