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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Cell Microbiol. 2009 Mar 18;11(7):1128–1150. doi: 10.1111/j.1462-5822.2009.01316.x

Figure 2.

Figure 2

Transcriptional profiling of intracellular Francisella (A) Representative chromatograms of total RNA isolated from either uninfected or Schu S4-infected BMMs at 0, 1, 2, 4, 8, 12, 16 and 24 h p.i., as described in the Materials and Methods section. The progressive increase in bacterial ribosomal RNAs over time mirrors the replication kinetics of intracellular bacteria (Fig. 1A). (B) Principal Component Analysis (PCA) plot showing the clustering of microarray data samples according to independent biological replicates of the same time points. PCA was performed using the Partek Genomics Suite software v6.3, as described in the Materials and Methods section. (C) Numbers of Francisella genes significantly up- or down-regulated at the various times p.i. Data from time zero samples was used as a baseline to determine genes whose mRNA levels varied by more than two fold. (D) Grouping of Francisella genes significantly up- or down-regulated according to their mRNA change index, i.e. the numbers of time points analyzed when expression of a given gene was significantly altered. Data from time zero samples was used as a baseline to determine genes whose mRNA levels varied by more than 2 fold. Numbers above brackets indicate the sums of genes in the included categories.