Table 1.
F. tularensis Schu S4 genes with altered intracellular expression previously shown to be involved in intracellular growth and/or virulence
| Locus tag | Gene name | Gene product | Phenotypic defect of transposon insertion mutants | Reference |
|---|---|---|---|---|
| Upregulated genes | ||||
| FTT0027c | fslC | Diaminopimelate decarboxylase | attenuation in mice (F. novicida) | (Weiss et al., 2007) |
| FTT0028c | fslB | Multidrug resistance efflux pump | attenuation in mice (F. novicida) | (Weiss et al., 2007) |
| FTT0029c | fslA | siderophore synthase | attenuation in mice (LVS) | (Su et al., 2007) |
| FTT0072 | sdhC | succinate dehydrogenase cytochrome b566 | attenuation in mice (F. novicida) | (Weiss et al., 2007) |
| FTT0113 | deoB | phosphopentomutase | attenuation in mice (F. novicida) | (Weiss et al., 2007) |
| FTT0209 | manganese ABC transporter | attenuation in mice (LVS) | (Su et al., 2007) | |
| FTT0296 | pcp | pyrrolidone-carboxylate peptidase | attenuation in mice (F. novicida) | (Weiss et al., 2007) |
| FTT0326 | rplD | LSU ribosomal protein L4P | attenuation in mice (F. novicida) | (Weiss et al., 2007) |
| FTT0356 | htpG | chaperone HtpG | attenuation in mice (F. novicida) | (Tempel et al., 2006, Weiss et al., 2007) |
| FTT0407 | gcvT | aminomethyltransferase | attenuation in mice (F. novicida) | (Weiss et al., 2007) |
| FTT0408 | gcvH | glycine cleavage system H protein | attenuation in mice (F. novicida) | (Weiss et al., 2007) |
| FTT0409 | gcvP1 | glycine dehydrogenase | attenuation in mice (F. novicida) | (Weiss et al., 2007) |
| FTT0413 | glgB | 1,4-alpha-glucan branching enzyme | intracellular growth defect and attenuation in mice (LVS) | (Maier et al., 2007, Su et al., 2007) |
| FTT0414 | pgm | phosphoglucomutase | attenuation in mice (F. novicida) | (Weiss et al., 2007) |
| FTT0503c | sucD | succinyl-CoA synthetase alpha chain | attenuation in mice (LVS) | (Su et al., 2007) |
| FTT0504c | sucC | succinyl-CoA synthetase beta chain | attenuation in mice (LVS and F. novicida) | (Su et al., 2007, Tempel et al., 2006) |
| FTT0610 | endonuclease | intracellular growth defect (LVS) | (Maier et al., 2007) | |
| FTT0624 | clpP | ATP-dependent protease ClpP | attenuation in mice (LVS) | (Su et al., 2007) |
| FTT0625 | clpX | ATP-dependent protease ClpX | attenuation in mice (LVS) | (Su et al., 2007) |
| FTT0626 | lon | Lon protease | attenuation in mice (LVS) | (Su et al., 2007) |
| FTT0667 | isochorismatase | attenuation in mice (F. novicida) | (Weiss et al., 2007) | |
| FTT0708 | MFS superfamily transporter | attenuation in mice (LVS) | (Su et al., 2007) | |
| FTT0884c | aspC1 | aspartate aminotransferase | attenuation in mice (LVS) | (Su et al., 2007) |
| FTT0937c | bioB | biotin synthase | attenuation in mice (F. novicida) | (Weiss et al., 2007) |
| FTT0945 | para-aminobenzoate synthetase component | intracellular growth defect and attenuation in mice | (Maier et al., 2007, Weiss et al., 2007) | |
| FTT1117c | isochorismatase | attenuation in mice (LVS) | (Su et al., 2007) | |
| FTT1124 | metN | ABC transporter ATP-binding protein | intracellular growth defect (LVS) | (Maier et al., 2007) |
| FTT1125 | metIQ | ABC transporter permease | intracellular growth defect and attenuation in mice (LVS) | (Maier et al., 2007, Su et al., 2007) |
| FTT1269c | dnaK | chaperone DnaK | attenuation in mice (F. novicida) | (Tempel et al., 2006) |
| FTT1344 | pdpA | pathogenicity determinant protein PdpA | attenuation in mice (F. novicida) | (Weiss et al., 2007) |
| FTT1356c | iglD | intracellular growth protein IglD | attenuation in mice (F. novicida) | (Weiss et al., 2007) |
| FTT1357c | iglC | intracellular growth protein IglC | intracellular growth defect and attenuation in mice | (Su et al., 2007, Weiss et al., 2007) |
| FTT1358c | iglB | intracellular growth protein IglB | intracellular growth defect and attenuation in mice | (Su et al., 2007, Weiss et al., 2007) |
| FTT1359c | iglA | intracellular growth protein IglA | intracellular growth defect and attenuation in mice | (Su et al., 2007, Weiss et al., 2007) |
| FTT1360c | pdpD | pathogenicity determinant protein PdpD | attenuation in mice (F. novicida) | (Weiss et al., 2007) |
| FTT1392 | bvgA | Bvg accessory factor | attenuation in mice (FTN_1355; F. novicida) | (Kraemer et al., 2009) |
| FTT1676 | hypothetical membrane protein | attenuation in mice (LVS) | (Su et al., 2007) | |
| FTT1696 | groEL | chaperonin GroEL | attenuation in mice (F. novicida) | (Weiss et al., 2007) |
| FTT1769c | clpB | chaperone ClpB | intracellular growth defect and attenuation in mice | (Gray et al., 2002, Meibom et al., 2008, Su et al., 2007, Tempel et al., 2006, Weiss et al., 2007) |
| Downregulated genes | ||||
| FTT0051 | rbfA | ribosome binding factor A | attenuation in mice (F. novicida) | (Weiss et al., 2007) |
| FTT0138 | secE | protein translocase subunit SecE | attenuation in mice (LVS) | (Su et al., 2007) |
| FTT0154 | xerD | integrase/recombinase XerD | attenuation in mice (F. novicida) | (Weiss et al., 2007) |
| FTT0584 | hypothetical protein | attenuation in mice (F. novicida) | (Weiss et al., 2007) | |
| FTT0805 | capB | capsular polyglutamate biosynthesis protein | intracellular growth defect and attenuation in mice | (Maier et al., 2007, Su et al., 2007, Weiss et al., 2007) |
| FTT0806 | capC | capsular polyglutamate biosynthesis protein | intracellular growth defect and attenuation in mice | (Maier et al., 2007, Su et al., 2007, Weiss et al., 2007) |
| FTT1234 | choloylglycine hydrolase | intracellular growth defect in HepG2 cells (Schu S4) | (Qin et al., 2006) | |
| FTT1416c | hypothetical lipoprotein | attenuation in mice (LVS) | (Su et al., 2007) | |
| FTT1442c | rpoA2 | RNA polymerase alpha chain | attenuation in mice (LVS) | (Su et al., 2007) |
| FTT1471c | deaD | ATP-dependent RNA helicase | attenuation in mice (LVS) | (Su et al., 2007) |