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. Author manuscript; available in PMC: 2010 Oct 20.
Published in final edited form as: Neuroscience. 2009 Jul 25;163(3):920–932. doi: 10.1016/j.neuroscience.2009.07.016

Figure 2.

Figure 2

Examples of cortical synapses that are immuno-stained for NR2A (A) or NR2B (B) subunits. Dense DAB accumulation is often encountered over postsynaptic densities (arrows) of spines (S1* in A and B) that receive synaptic contacts from vesicle filled profiles (t1 in A). Chromagen diffusion into the spine cytoplasm is encountered at varying degrees. DAB label is easily differentiable from electron dense appearance of unlabeled synapses (S2 in A and B). No qualitative differences are noted for NR2A or NR2B staining. Scale bar= 250nm.