Table 1.
S. cerevisiae strains, plasmids and oligonucleotides used
| Strain, plasmid, oligonucleotide | Description | Source or reference |
|---|---|---|
| S. cerevisiae | ||
| (1) BY4741 | MATahis3Δ1leu2Δ0met15Δ0ura3Δ0 | EUROSCARF |
| (2) BY4741oar1Δ1a | ykl055c::kanMX | EUROSCARF |
| yPLM372 | Expressing peroxisomal catalase A (Cta1p) from pPLM187 | This study |
| yPLM382 | Expressing mitochondrial Coq3p-FabG1 from pPLM189 | This study |
| yPLM392 | Expressing mitochondrial Coq3p-FabG2 from pPLM190 | This study |
| yPLM402 | Expressing mitochondrial Coq3p-FabG3 from pPLM191 | This study |
| yPLM412 | Expressing mitochondrial Coq3p-FabG4 from pPLM192 | This study |
| yPLM422 | Expressing mitochondrial Coq3p-FabG5 from pPLM193 | This study |
| yPLM432 | Expressing mitochondrial Oar1p from pPLM188 | This study |
| Plasmid | ||
| (3) pBluescript KS III | pKS cloning vector | Stratagene |
| pPLM833 | pKS:Rv1483 mitochondrial fabG1 in pBluescript | This study |
| pPLM823 | pKS:Rv1350 mitochondrial fabG2 in pBluescript | This study |
| pPLM863 | pKS:Rv2002 mitochondrial fabG3 in pBluescript | This study |
| pPLM783 | pKS:Rv0242c mitochondrial fabG4 in pBluescript | This study |
| pPLM963 | pKS:Rv2766c mitochondrial fabG5 in pBluescript | This study |
| (4) YEp352 | URA3-marked multicopy plasmid | Hill et al. (1986) |
| (5) pPLM1874 | CTA1 behind its promoter (pYE352:CTA1) | Filppula et al. (1995) |
| pPLM1885 | OAR1 behind the CTA1 promoter (pYE352:OAR1) | Chen et al. (2009) |
| (6) pPLM625 | COQ3-QOR fusion behind the CTA1 promoter | Torkko et al. (2001) |
| pPLM1896 | COQ3-fabG1 fusion behind the CTA1 promoter | This study |
| pPLM1906 | COQ3-fabG2 fusion behind the CTA1 promoter | This study |
| pPLM1916 | COQ3-fabG3 fusion behind the CTA1 promoter | This study |
| pPLM1926 | COQ3-fabG4 fusion behind the CTA1 promoter | This study |
| pPLM1936 | COQ3-fabG5 fusion behind the CTA1 promoter | This study |
| Oligonucleotide | ||
| Rv1483 MLS-FabG1 F | 5′-TTATCCATGGCTGCCACAGCCACTGAAGGGGC-3′ | This study |
| Rv1483 FabG1 R | 5′-TATTAAGCTTCAGTGGCCCATACCCATGCC-3′ | This study |
| Rv1350 MLS-FabG2 F | 5′-TTATCCATGGCGTCATTGCTGAACGCTC-3′ | This study |
| Rv1350 FabG2 R | 5′-TATACTCGAGTCATATGAACCGGCCGCCAG-3′ | This study |
| Rv2002 MLS-FabG3 F | 5′-TTATCCATGGCTGGACGGTTGATAGGAAAGG-3′ | This study |
| Rv2002 FabG3 R | 5′-TATTAAGCTTACGTCACCCATTCCGGCTGCG-3′ | This study |
| Rv0242c MLS-FabG4 F | 5′-TTATCCATGGCTCCCAAGCGTTCGTCCGATC-3′ | This study |
| Rv0242c FabG4 R | 5′-TATTAAGCTTCACGCGCCGATCATGGCCTGGC-3′ | This study |
| Rv2766c MLS-FabG5 F | 5′-TTATTATCATGACCTCACTGGATCTGACC-3′ | This study |
| Rv2766c FabG5 R | 5′-TTATTATCATGACCTCACTGGATCTGACC-3′ | This study |
aThe numbers in superscript following the designation of the strains refer to their parental genotypes, e.g., BY4741oar1Δ1 was derived from (1) BY4741. The same principle applies to plasmids