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Enzyme activities in a yeast oar1Δ mutant expressing FabG4
| Substrate | 3-Oxoacyl-thioester reductase activity (nmol/mg protein × min−1) | |
|---|---|---|
| NAD(H) | NADP(H) | |
| 2-trans-hexenoyl-CoA | 210 ± 20a | 3.5 ± 0.3 |
| 2-trans-decenoyl-CoA | 250 ± 80 | Not tested |
aThe values are means ± standard deviations (n = 3) and represent averages of measurements using extracts obtained from three independent yeast cultures following overnight oleic acid induction. The yeast strain used was yPLM41
