Fig. 2.

Immunoblot of commercial and self prepared extract with a human serum from a non-allergic individual. Commercial and self prepared extracts were separated using SDS-PAGE, blotted and developed with the human serum. The following marker and samples were applied: lane 1 molecular weight marker, lane 2 commercial extract A, lane 3 commercial extract B, lane 4 commercial extract C, lane 5 commercial extract D, lane 6 self prepared extract from German Simmental, lane 7 self prepared extract from German Brown, lane 8 self prepared extract from Holstein-Friesian, lane 9 self prepared extract from German Red Pied. The following amounts of protein were applied: lanes 2–9: 20 μg