Figure 3.
UPR expression in arterial endothelium. Gene and protein expression of tripartite branches of UPR signaling: (A) ATF6α branch (B) IRE1α branch (C) PERK branch in aortic arch, AA, normalized to descending thoracic aorta, DT, for each paired sample based on their animal origin. Gene (n=6–10 paired samples) and protein (n=10–12 paired samples) expression was normalized to GAPDH and β-actin, respectively. Values > 1.0 indicate higher expression in AA. Data represent mean±SEM. N.S.: non-significant. *p≤0.05 one-sample, one sided, paired Wilcoxon test.