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. Author manuscript; available in PMC: 2010 Jul 31.
Published in final edited form as: Circ Res. 2009 Jul 9;105(3):295–303. doi: 10.1161/CIRCRESAHA.109.194613

Figure 4.

Figure 4

Preparation of LDL receptor KO mice with a macrophage-specific SMS2 deficiency. LDLr−/− animals were lethally irradiated. After3 hours, half of them were transplanted with SMS2−/− mouse bone marrow cells (SMS2−/−→LDLr−/−), and the other half with WT ones (WT→LDLr−/−). Panel A, genotype determination of mouse peripheral cells. Genomic DNA was extracted from white blood cells of the same mouse, before and after irradiation. The genotypes of SMS2 were determined with PCR, as described in “Methods.” WT mice had a 500 bp PCR product, and KO ones a 300 bp PCR product; Panel B, macrophage SMS2 mRNA levels measured by real time-PCR; Panel C, macrophage SMS1 mRNA levels measured by real time-PCR; Panels D–F, macrophage SM, ceramide, and PC measurement; Panel G, macrophage plasma membrane SM measurement (lysenin-mediated cell lysis assay21,26). Values in panel B-G are mean ± SD, N=5. *P<0.05.