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. Author manuscript; available in PMC: 2009 Sep 21.
Published in final edited form as: Nat Cell Biol. 2008 Sep;10(9):1062–1068. doi: 10.1038/ncb1765

Figure 4.

Figure 4

Talin depletion impairs FAK-Tyr 397 phosphorylation upon adhesion to fibronectin. (a) Western blots showed defects in adhesion-dependent FAK-Tyr 397 phosphorylation in talin-deficient cells. Control, talin2 siRNA, and talin1H and talin2 siRNA co-transfected cells were plated on fibronectin-coated culture dishes for the indicated time before lysis. (b) Profile of FAK-Tyr 397 phosphorylation levels with adhesion to fibronectin. pTyr 397: FAK ratio of control cells at 120 min was set at 1 arbitrary unit (AU) and the values of other time points were adjusted proportionally. Data are mean ± s.e.m., n = 3. (c) Activation profile of SFK with adhesion to fibronectin. pTyr 416: Src ratio increase of 10 min above the suspension sample in control cells was set at 1 AU and other time points were adjusted proportionally. Data are mean ± s.e.m., n = 3. (d–f) Immunostaining for phospho-FAK (pTyr 397) in control (d), talin2-deficient (e) and talin1H-expressing cells (f). Cells were fixed 20 min (d, e) and 90 min (f) after plating. The pTyr 397 signal was lost in talin2-deficient and talin1H-expressing cells. Scale bars are 10 μm.