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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Exp Hematol. 2009 May 15;37(8):889–900. doi: 10.1016/j.exphem.2009.05.004

Fig. 1. Protein-protein interaction occurs between GATA-1 and Stat3.

Fig. 1

A) Western blot (WB) analysis was performed to determine pStat3 and GATA-1 protein levels (see materials and methods). Phosphorylated Stat3 (pStat3) antibody (left blot) and the nuclear proteins shown were used to demonstrate the presence of an 89kDa band in K562, HeLA and mouse erythroleukemia (MEL) cells. Membranes were stripped and probed with total Stat3 (t-Stat3) antibody as a loading control. WB with GATA-1 antibody (right blot) demonstrated its presence in K562 and MEL cells extracts but not in non-hematopoietic HeLa cells. Actin was used as a loading control. B) Immunoprecipitation (IP) reactions were performed with GATA-1 antibody or pre-immune serum (Pre-IS) followed by WB with pStat3 antibody. C) Nuclear proteins from the indicated cells were immunoprecipitated with pStat3 antibody followed by WB with GATA-1 antibody.