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. 2009 Sep 30;4(9):e7209. doi: 10.1371/journal.pone.0007209

Figure 5. Transcriptional regulation activity of GmPHDs in protoplast assay.

Figure 5

(A) Effects of the GmPHDs on reporter gene expression as revealed by relative LUC activity. The GAL4 DNA-binding domain (BD) and VP16 were used as negative and positive controls respectively. “**” indicate highly significant difference (P<0.01) compared to BD value. (B) Effects of the GmPHDs on VP16-mediated LUC gene expression. The Arabiodpsis Dof23 was used as a non-interactive control. (C) Effects of various domains of the GmPHD2 on VP16-mediated LUC gene expression. For (B) and (C), “*” and “**” indicate significant difference (P<0.05 and P<0.01 respectively) compared to VP16 value.