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. 2009 Sep 28;4(9):e7202. doi: 10.1371/journal.pone.0007202

Figure 4. Comparison of spectra recorded for the U1 snRNP isolated from HeLa cells and a recombinant human U1 snRNP complex reconstituted in vitro.

Figure 4

(a) The high m/z region of recombinant U1 snRNP reconstituted with all subunits except U1-C and with truncated Sm-B/B', shows remaining stripped complexes after loss of U1-A, Sm-E and Sm-F (or Sm-G* - masses of Sm-F and tagged recombinant Sm-G* cannot be distinguished (table S7) (b) U1 snRNP from HeLa cells showing predominantly loss of Sm-B/B'. Inset shows the low m/z region containing an extended distribution of charge states from dissociated full-length Sm-B/B'. Solution and MS conditions (a) 850 mM ammonium acetate. LCT: capillary: 1.5 kV, cone: 200 V, extractor: 75 V, source readback: 8.4 mbar, ToF readback: 2.1×10−6 mbar. (b) 200 mM ammonium acetate. QToF2: capillary: 1.5 kV, cone: 200 V, extractor: 0 V, collision cell voltage: 160 V, source transfer region readback: 7.1×10−3 mbar, ToF readback: 1.3×10−6 mbar.