(A) 107 wild-type, CCN2 over-expressing, and CCN2 shRNA-expressing Panc-1 clones were subcutaneously implanted in nu/nu mice. Tumor volumes were monitored weekly using calipers. Panc-1 + CCN2 shRNA (pool) curve is included from Figure 2A for visual comparison purposes only. Data are mean ± SEM with at least 5 mice per group; *p<0.05 by ANOVA of AUCs.
(B) 107 wild-type, CCN2 over-expressing, or CCN2 shRNA-expressing Panc-1 cells were orthotopically implanted in nu/nu mice. Tumor volume was monitored by uptake of intravenously administered 18F-deoxyglucose (FDG) using positron emission tomography (PET). Representative maximum intensity projections are shown, with regions of high FDG uptake highlighted. G = Harderian glands, H = heart, K = kidney, B = bladder, T = tumor.
(C) Survival plot of mice bearing orthotopic pancreatic tumors. Data from 3-9 mice per group; p-values indicate comparison with mice bearing wild-type tumors by log-rank test.