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Electrophoretic fractionation of a BSA raw digest containing tween-20 detergent. A) The raw sample shows a strong interference in the mass spectra from the detergent which is completely absent after electrophoresis. B) BSA peptides are fractionated into two complementary fractions defined by the pH of the electrophoresis running buffer. The total number of detected BSA peptides increases from 29 in the detergent containing raw sample to 42 after fractionation.
