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Extracellular calcium regulates lateral pseudopod formation in the absence of cAMP (A) and in a spatial gradient of cAMP (B). Pseudopods were identified and counted as described in Methods section. The mean is presented for 10 cells at each concentration. The standard deviation was less than 50% of the mean for all data points ≥ 2.4, and no greater than the mean for all data points ≤ 1.3.
