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. Author manuscript; available in PMC: 2009 Dec 15.
Published in final edited form as: J Immunol. 2009 Jun 15;182(12):7343–7347. doi: 10.4049/jimmunol.0804295

FIGURE 3.

FIGURE 3

B cell-intrinsic CD19 signal is required for MZ B cell differentiation. Spleens from CD19ko, CD45.2 recipients were analyzed 7 days after adoptive transfer of B cells from CD45.1 WT or CD19ko (KO) donors. A, Spleen sections were stained for B cells (anti-IgM; red), donor cells (anti-CD45.1; green), MMM (anti-SIGLEC-1, which delineate the inner boundary of the MZ; blue), and T cells (anti-CD4; cyan). Arrows indicate that MZ B cells are reconstituted in mice receiving WT B cells but not CD19ko B cells. B and C, B cells in the spleens of CD19ko, CD45.2 recipient mice 7 days after adoptive transfer of CD45.1 B cells from WT or CD19ko mice were analyzed by flow cytometry. B, CD45.1 donor B cells were analyzed for the distribution of mature (AA4.1-) MZ and FO phenotypes. C, all mature B cells in the MZ and FO compartments in recipient mice were analyzed for donor and recipient B cells. The figures highlighted in light blue are the ratios of the frequencies of donor cells in MZ B cells (middle column) or FO B cells (right column) to the frequencies of donor cells in total B cells. D and E, The ratios of the numbers of donor B cells in the inner and outer halves of follicles, as illustrated (D), of the indicated recipient mice that received either WT or CD19ko B cells were calculated using image analysis software (E). *, p < 0.05; **, p < 0.005; ***, p < 0.001.