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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: J Immunol. 2009 Aug 10;183(5):2966–2973. doi: 10.4049/jimmunol.0900442

Figure 5.

Figure 5

Effect of TBS mutationd on canonical NF-κB activation. A, Whole cell lysates from M12.4.1 B cell transfectants stimulated for indicated times with agonistic anti-mouse or human CD40 mAb or isotype control mAbs were analyzed by immunoblotting for total IκBα or actin. B. Quantification of IκBα degradation was performed by measuring total IκBα and actin bands with a low-light imaging system. The amount of IκBα was normalized to the corresponding actin band. These values were then normalized to the 0 time (0 min. IκBα/actin), which was set as 100%. Black solid lines represent IκBα degradation induced by anti-hCD40 agonistic mAb, grey dashed lines represent IκBα degradation induced by anti-mCD40 agonistic mAb. Data shown are representative of three or more experiments performed with two or more clones of each transfectant.