Skip to main content
. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: J Immunol. 2009 Aug 10;183(5):2966–2973. doi: 10.4049/jimmunol.0900442

Figure 6.

Figure 6

Effect of TBS mutation on total NF-κB activation. M12 B cells expressing either hCD40, hCD40LMP1, hCD40LMP1AEDL, or hCD40ADD were transiently transfected with a NF-κB firefly reporter plasmid and a renilla control plasmid, stimulated with α-mouse or human CD40 or isotype control Abs for 6h. Values from each sample were normalized for transfection efficiency by dividing the renilla luciferase activity by the firefly luciferase activity in each sample. The stimulation index for each receptor was determined by dividing the normalized values of each sample as: (αhCD40 mAb stimulated- isotype control mAb stimulated)/(αmCD40 mAb stimulated- isotype control mAb stimulated). Data shown are representative of three or more experiments performed with two or more clones of each transfectant.