Table 3.
Tajima’s relative rate test for the comparison of evolutionary distance between GLI paralogs in different species using the Drosophila Ci as an outgroup.
| Evolutionary Distance | x2 | df | p |
|---|---|---|---|
| Human | |||
| GLI1 vs GLI2 | 6.43 | 1 | 0.011* |
| GLI1 vs GLI3 | 5.24 | 1 | 0.022* |
| GLI2 vs GLI3 | 0.20 | 1 | 0.652 |
| Mouse | |||
| GLI1 vs GLI2 | 9.19 | 1 | 0.002* |
| GLI1 vs GLI3 | 7.01 | 1 | 0.008* |
| GLI2 vs GLI3 | 0.17 | 1 | 0.676 |
| Rat | |||
| GLI1 vs GLI2 | 3.33 | 1 | 0.068 |
| GLI1 vs GLI3 | 3.57 | 1 | 0.059 |
| GLI2 vs GLI3 | 0.54 | 1 | 0.463 |
| Frog | |||
| GLI1 vs GLI2 | 0.30 | 1 | 0.581 |
| GLI1 vs GLI3 | 3.21 | 1 | 0.073 |
| GLI2 vs GLI3 | 5.80 | 1 | 0.016* |
| Tetraodon | |||
| GLI1 vs GLI2 | 3.42 | 1 | 0.064 |
| GLI1 vs GLI3 | 2.14 | 1 | 0.143 |
| GLI2 vs GLI3 | 0.31 | 1 | 0.579 |
| Zebrafish | |||
| GLI1 vs GLI2 | 1.03 | 1 | 0.310 |
| GLI1 vs GLI3 | 0.17 | 1 | 0.680 |
| GLI2 vs GLI3 | 0.01 | 1 | 0.920 |
| Fugu | |||
| GLI1 vs GLI2 | 1.27 | 1 | 0.259 |
| GLI1 vs GLI3 | 0.32 | 1 | 0.574 |
| GLI1 vs GLI3 | 2.04 | 1 | 0.153 |
P-value with “*” symbol represents the situation where GLI1 (human and mouse) and GLI2 (frog) evolves significantly (p < 0.05) faster than the counterpart.