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. 2009 Sep;128(1):58–68. doi: 10.1111/j.1365-2567.2009.03071.x

Figure 2.

Figure 2

Inhibition of coculture activation reveals differential regulation of cytokine production. Cocultures of endothelial cells (EC) and monocytes were formed, stimulated with lipopolysaccharide (LPS; 10 ng/ml) for 24 hr, and their production of cytokines determined by enzyme-linked immunosorbent assay (ELISA). (a) Both interleukin-1 receptor anatagonist (IL-1ra) and hydrocortisone reduced the release of IL-6 from the LPS-treated cocultures. (b) Hydrocortisone, but not IL-1ra, reduced the release of IL-1β from the LPS-treated cocultures. (c) Both IL-1ra and hydrocortisone reduced the release of CXCL8 from the LPS-treated cocultures. M indicates monocytes, EC indicates EC and EC + M indicates an EC/monocyte coculture. HC indicates hydrocortisone. Data are ± SEM (n = 4), with each experiment using cells from independent donors. **P < 0·01, and *P < 0·05, analysed using one-way anova with Tukey’s post-test. LD indicates the limit of detection of the ELISA.