Figure 7.

Regulation of interleukin-17 (IL-17) release. The ability of cell-free supernatants from endothelial cells treated with either buffer or lipopolysaccharide (LPS) to support IL-17 production was determined. Lymphocytes were stimulated with anti-CD3 in coculture with monocytes and 100 ng/ml LPS, and the resulting IL-17 generation was measured by enzyme-linked immunosorbent assay. Addition of media from endothelial cells (EC) treated with buffer alone caused suppression of IL-17 generation from this activated coculture, whereas media from EC activated with 1 ng/ml LPS did not suppress IL-17 production. EC media were added at a 1 : 2 dilution. Data are ± SEM. Effects of supernatants from five EC donors, each treated with buffer or LPS, were tested on one monocyte/lymphocyte donor. *P < 0·05, analysed by t-test.