Fig. 4.

Time and 2D-space resolved fluorescence analysis of a dark spot and its blue luminescent halo. In vivo luminescence of a luminescent ring surrounding a growing dark spot (detected at 450 nm, excitation at 350 nm, 0.5 mm spatial resolution); luminescence maps (Left) in false colors, recorded at the indicated times, with contour lines at specified absolute fluorescence intensities, and corresponding vertical projections (Right) as cut (through) near the center of a dark spot (see red frame) (Bottom).