Fig. 6.
tRNAPhe and imG-14 recognition mechanism by PhTYW2. (A) Overview of the Trm5·tRNACys complex structure. (B) Overview of the PhTYW2·tRNA docking model. The tRNA molecules are colored pink. The electrostatic potential distribution is presented on the contact surface of PhTYW2, with positively and negatively charged regions colored blue and red, respectively. The contact surface and the electrostatic potential were calculated by PyMOL (http://pymol.sourceforge.net/). (C and D) Active sites of the Trm5·tRNACys complex and the PhTYW2 docking model. The G37 and imG-14 residues and AdoMet are shown by stick models. Hydrogen bonds are indicated by black dashed lines. The acceptor atoms of the chemical groups transferred from AdoMet are indicated by arrows.