Fig. 5.

Oligomerization of TβRII-GFP on the cell membranes with the increased expression time. (A) Typical images (after background subtraction) showing the density and size of TβRII-GFP monomers, oligomers and aggregates on cell membranes imaged at 4 h (Left), 8 h (Center), and 16 h (Right) after transfection. (Scale bar, 2 μm.) (B) The histogram of the fluorescence intensities of individual diffraction-limited fluorescent spots (n = 530) on living cell membranes imaged at 8 h after transfection. Correlation coefficient of the Gaussian fitting is 0.995.