Fig. 3.

Recombination protein RAD51 and replication protein A (RPA) preferentially associate with particular telomeres in a cluster. (A and B) IF analyses of VA13 cells fixed 24 h posttransfection with BFP-ICP0* (not represented in the figures) revealing the co-localization of anti-RAD51 (A) or anti-RPA (B) staining (both red) with individual telomeres, as detected by anti-TRF2 antibody (green), in e-APBs. 24% (n = 20) and 35% (n = 20) of RAD51 and RPA nuclear foci, respectively, were found associated with TRF2 in e-APBs. Localization of the same proteins in unperturbed APBs in non-transfected cells is also shown. Scale bars, 2 μm. (C) Models of native PML and native and e-APB structures. Infiltration by ICP0* leads to displacement of proteins from the core of the PML scaffold but preserves the association of telomeres with the outer layer of the body.