Table 1.
Quantification of the number of telomeres associated with APBs in different cell lines
| Cell line | # ICP0* foci | # TRF2 foci | % TRF2 associated with ICP0* |
|---|---|---|---|
| GM847 | 42.0 ± 3.1 | 80.8 ± 4.1 | 46.1 ± 2.1 |
| VA13 | 35.9 ± 3.0 | 90.3 ± 5.1 | 40.3 ± 2.6 |
| VA13 + telomerase | 29.1 ± 1.2 | 74.4 ± 2.4 | 38.2 ± 0.9 |
| U2OS | 38.5 ± 2.4 | 72.1 ± 4.4 | 31.3 ± 2.0 |
| SAOS2 | 29.4 ± 1.9 | 58.3 ± 3.9 | 20.0 ± 3.6 |
| VA13-C3-cl6 | 42.5 ± 2.6 | 108.3 ± 3.3 | 8.7 ± 1.5 |
Different cell lines (see Fig. 2A) were transfected with CFP-ICP0* to create e-APBs and analyzed by immunofluorescence using anti-TRF2 antibodies 24 h post transfection. Average statistics with SEM are reported based on the analysis of 20 flat projections of 3D nuclear images per cell line. The percentage of TRF2 foci associated with ICP0* was corrected for the level of random colocalization of TRF2 with ICP0* due to the flattening projections of 3D stacks as determined in HT1080 cells (7.6% ± 0.9%).