Fig. 2.

c-MYC directly regulates the pri-mir-17–92 upon estrogenic stimulation. (A) Schematic representation of the mir-17–92 cluster genomic region. Both the c-MYC binding site and a putative ERE half site are indicated. (B) MCF-7 cells were maintained in estrogen-free medium for 3 days (starvation) and then either left untreated (vehicle) or treated with 10 nM E2 for 3 h after which ChIP was performed, followed by real time PCR. The c-MYC interaction site genomic region is presented. (C) After starvation, MCF-7 cells were treated with E2 for 12 h before ChIP.