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ERα is directly regulated by miRNA-3′-UTR interaction. (A) Representation of the 4 different lengths of ERα 3′-UTR cloned in the pMIR-REPORT lucifearse vector and miRNAs interaction sites. (B) Luciferase activity from cells cotransfected with pre-miR-18a, pre-miR-19b, pre-miR-20b, pre-miR-n.c., and different lengths of DNA fragments corresponding to the ERα 3′-UTR. Firefly luciferase was normalized for transfection levels to Renilla luciferase as indicated in experimental procedures.
