Fig. 5.
CENP-A recruitment to sites of DNA damage requires its centromere-targeting domain (CATD) and recruits centromeric nucleosome-associated factors CENP-N and CENP-U. (A) Schematic of CENP-A/H3 chimeric proteins and frequencies at which each is recruited to DNA damage foci. (B–E) Human HCT116 cells were transiently transfected. DNA damage was induced by laser targeting, and frequencies of focal accumulation at site of DNA damage were measured. (B) GFP-H3CATD. (C) GFP-CENP-N. (D) GFP-CENP-U. (E) GFP-CENP-T.