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. 2009 Sep 1;106(37):15813–15818. doi: 10.1073/pnas.0907722106

Fig. 2.

Fig. 2.

Nod2 is required to induce bactericidal activity in crypt secretions of the terminal ileum. (A) Crypts were isolated from the terminal ileum of wild-type and Nod2-deficient mice. Granules within Paneth cells were stained with 0.25% amido black. (B) We stimulated 2,000 crypts from wild-type and Nod2-deficient mice with CCH (1 μM), MDP, MDPDD, and MDPLL (1 mg/mL), and secretions were subjected to a bactericidal activity assay against E. coli. (C) Crypts from wild-type and Nod2-deficient mice (Left) or from wild-type and Rip2-deficient mice (Right) were stimulated with CCH as in (B), and secretions were tested for bactericidal activity against E. coli, S. typhimurium, and Listeria monocytogenes. Error bars represent standard deviation. Data are representative of 5 independent experiments.