Skip to main content
. 2009 Aug 31;106(37):15819–15824. doi: 10.1073/pnas.0901216106

Fig. 3.

Fig. 3.

miR-147 is induced by stimulation of multiple TLRs. (A) Dose-dependent induction of miR-147 by poly(I:C) and LPS. Peritoneal macrophages were treated with PAM3CSK4, poly(I:C) or LPS at the indicated concentrations for 6 h (PAM3CSK4 and LPS) or 9 h (poly(I:C)). Real-time PCR was performed. Values are presented as mean ± SD from triplicate wells. ###, P < 0.001 compared to cells treated with 10 μg/mL PAM3CSK4. ***, P < 0.001 when compared to cells treated with 50 μg/mL poly(I:C). (B) LPS treatment rapidly induces IFN β expression. Peritoneal macrophages were treated with 1 μg/mL LPS for 0, 30, 60, and 120 min. The expression of IFN β was determined by real-time PCR analysis. (C and D) LPS-induced miR-147 expression requires de novo protein synthesis. Peritoneal macrophages were pretreated with or without 5 μg/mL cycloheximide (CHX) for 1 h. CHX was then removed and the cells were cultured with or without 1 μg/mL LPS for 3 h. Real-time PCR was performed to determine the levels of pri-mir-147 (C) and IL-1β (D). Values are presented as mean ± SD from four wells. *, P < 0.05 compared to cells treated with LPS alone. (E) Poly(I:C), but not PAM3CSK4-induced miR-147 expression requires de novo protein synthesis. Peritoneal macrophages were treated as in (C), except replacing LPS with 1 μg/mL PAM3CSK4 or 5 μg/mL poly(I:C).