Table 1.
Data collection and refinement statistics
| BToV HE | BToV HE with ligand | PToV HE S46A | PToV HE S46A with ligand | |
|---|---|---|---|---|
| Space group | P212121 | P212121 | C2 | C2 |
| Cell dimension a, b, c (Å), α, β, γ (o) | 67.8, 113.4, 273.5 90.0, 90.0, 90.0 | 67.6, 112.9, 273.0 90.0, 90.0, 90.0 | 156.3, 104.5, 97.5 90.0, 95.8, 90.0 | 156.0, 103.7, 97.1 90.0, 96.0, 90.0 |
| Resolution (Å)* | 50–1.8 (1.89–1.80) | 50–2.0 (2.11–2.00) | 58–2.1 (2.21–2.10) | 48–2.8 (2.94–2.79) |
| Completeness (%) | 95.3 (86.3) | 99.5 (98.6) | 100 (100) | 94.3 (95.3) |
| No. unique reflections | 186,978 | 141,552 | 90,955 | 36,147 |
| Multiplicity | 5.9 (5.8) | 3.7 (3.7) | 3.8 (3.8) | 2.7 (2.6) |
| Rmerge (%) | 7.2 (48.5) | 7.5 (34.0) | 11.6 (72.1) | 8.6 (48.7) |
| I/σ | 14.4 (3.6) | 12.0 (3.8) | 8.4 (2.2) | 10.5 (2.0) |
| Rfactor/Rfree (%) | 18.9/21.8 | 17.8/20.9 | 17.1/20.4 | 19.3/24.3 |
| rmsd bond lengths (Å) | 0.012 | 0.013 | 0.015 | 0.008 |
| rmsd bond angles (°) | 1.4 | 1.4 | 1.6 | 1.2 |
| No. protein atoms | 11,419 | 11,419 | 8,463 | 8,463 |
| No. glycan units | 44 | 31 | 16 | 16 |
| No. Waters | 1,276 | 1,026 | 518 | 11 |
*Values in parentheses refer to the highest-resolution shell of data.